Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ba/F3 cell line transfected with mouse TLR4 /MD2 complex

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-P - Verified
ICFC, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume or 100 ?L?of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.⁸ Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.⁸ In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
Kanada S, et al. 2011. Blood 117:2211. PubMed
Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
Akashi S, et al. 2003. J. Exp. Med. 198:1035.

RRID

AB_2819836 (BioLegend Cat. No. 117617) AB_2819837 (BioLegend Cat. No. 117618)

Antigen Details

Structure: Type I transmembrane protein complex
Distribution: Monocytes, B cells and T cells
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.
Gene ID: 21898 View all products for this Gene ID
UniProt: View information about TLR4 MD2 Complex on UniProt.org

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