Purified anti-mouse TLR4 (CD284)/MD2 Complex Antib-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1344	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ba/F3 cell line transfected with mouse TLR4 /MD2 complex

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-P, WB - Verified
ICFC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.⁸ Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.⁸ In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
Kanada S, et al. 2011. Blood 117:2211. PubMed
Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
Akashi S, et al. 2003. J. Exp. Med. 198:1035.

Product Citations

Ricci-Azevedo R, et al. 2021. Front Immunol. 12:655371. PubMed
Suzuki M, et al. 2009. J Immunol. 182:6485. PubMed
Laplante P, et al. 2011. J Biol Chem. 286:42494. PubMed
Shimizu J, et al. 2022. Front Immunol. 13:903859. PubMed
Yang H, et al. 2009. PLoS One. 4:e6520. PubMed
Long C, et al. 2012. J Leukoc Biol. 92:667. PubMed
Iwata Y, et al. 2009. Am J Pathol. 175:649. PubMed
Mao A, et al. 2005. J Immunol. 175:5146. PubMed
Saxena A, et al. 2011. Cardiovasc Res. 91:350. PubMed
Sheng K, et al. 2010. J Immunol. 184:2863. PubMed
Suzuki H, et al. 2020. J Immunol. 205:811. PubMed
Bhattacharyya S, et al. 2007. Am J Physiol Gastrointest Liver Physiol. 293:G429. PubMed
Sheng K, et al. 2008. J Immunol. 181:2455. PubMed

RRID

AB_313788 (BioLegend Cat. No. 117601) AB_313789 (BioLegend Cat. No. 117602)

Antigen Details

Structure: Type I transmembrane protein complex
Distribution: Monocytes, B cells and T cells
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.
Gene ID: 21898 View all products for this Gene ID
UniProt: View information about TLR4 MD2 Complex on UniProt.org

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