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PE anti-mouse TLR4 (CD284)/MD2 Complex Antibody
产品名称:
PE anti-mouse TLR4 (CD284)/MD2 Complex Antibody
产品类别:
抗体
产品编号:
117605
产品应用:
117605
[价格]
规格 价格 库存
50µg ¥ 2568 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
  2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
  3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
  4. Kanada S, et al. 2011. Blood 117:2211. PubMed
  5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
  6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
  7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
  8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
Product Citations
  1. Souza-Fonseca-Guimaraes F, et al. 2012. J Immunol. 188:5850. PubMed
  2. Singh M 2014. J Lab Autom. 19:587. PubMed
  3. Takayanagi Y, et al. 2020. J Clin Med. 9:00. PubMed
  4. Kimura T, et al. 2015. J Immunol. 194:5426. PubMed
  5. Tanigawa T, et al. 2013. Innate Immunity. 19:115. PubMed
  6. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
  7. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  8. Nalbandian G, et al. 2005. J Immunol. 175:2666. PubMed
  9. Lim TJF, et al. 2020. J Exp Med. 217:00:00. PubMed
  10. Kanada S, et al. 2011. Blood. 117:2211. PubMed
RRID
AB_313792 (BioLegend Cat. No. 117605) AB_2205138 (BioLegend Cat. No. 117606)

Antigen Details

Structure
Type I transmembrane protein complex
Distribution

Monocytes, B cells and T cells

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about TLR4 MD2 Complex on UniProt.org

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