Ultra-LEAF™ Purified anti-mouse CD154 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Activated mouse Th1 clone D1.6

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding^3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 106515-106520).

Application References (PubMed link indicates BioLegend citation)

Lettesj? H, et al. 2000. J. Immunol. 165:4095. (IHC)
Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
Lawson BR, et al. 2007. J. Immunol. 178:5366.

RRID

AB_2813945 (BioLegend Cat. No. 106515) AB_2813946 (BioLegend Cat. No. 106516) AB_2813947 (BioLegend Cat. No. 106517) AB_2813948 (BioLegend Cat. No. 106518) AB_2813949 (BioLegend Cat. No. 106519) AB_2813950 (BioLegend Cat. No. 106520)

Antigen Details

Structure: TNF superfamily, 39 kD
Distribution: Activated CD4⁺ T cells
Function: T-B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
Gene ID: 21947 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们