Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume It is recommended that the reagent be titrated for optimal performance for each application.

Additional reported applications (for the relevant formats) include: immunohistochemical staining^1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding^3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 106515-106520).

1. Lettesj? H, et al. 2000. J. Immunol. 165:4095. (IHC)
2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.

1. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
2. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
3. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
4. Gengenbacher M, et al. 2014. MBio. 5:1262. PubMed
5. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
6. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
7. Danelli L, et al. 2015. Cancer Immunol Res. 3:85. PubMed
8. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
9. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
10. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
11. Iwata H, et al. 2013. J Immunol. 191:2978. PubMed
12. Chen C, et al. 2011. PLoS One. 6:e17712. PubMed
13. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
14. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
15. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed

Activated CD4⁺ T cells

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.