Purified anti-human IL-6 Antibody
- 产品名称：
- Purified anti-human IL-6 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 501204
- 产品应用：
- 501204
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-7- expressed, recombinant human IL-6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA, WB
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 ?g/ml is recommended.
It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Detection1-4: The biotinylated MQ2-39C3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MQ2-13A5 antibody (Cat. No. 5001102/501110) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1, Western blotting. The LEAF? purified MQ2-13A5 antibody (Endotoxin Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430501 to 430506) are specially developed and recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
- Gaines Das R, et al. 1993. J. Immunol. Meth. 160:147.
- Lesmeister MJ, et al. 2005. Reproductive Biology and Endocrinology 3:74.
- Product Citations
-
- Rodriguez-Salazar CA, et al. 2022. Viruses. 14:. PubMed
- RRID
- AB_2296206 (BioLegend Cat. No. 501204)
- Structure
- Neuropoietic cytokine family; 26 kD (Mammalian)
- Bioactivity
- Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
- Cell Sources
- T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
- Cell Targets
- T cells, B cells, hepatocytes, cholinergic neurons
- Receptors
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
- Regulation
- Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
- Gene ID
- 3569 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org
