Biotin anti-human IL-6 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7- expressed, recombinant human IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection - Quality tested
ICC?- Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/mL?are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1.0 - 4.0 ?g/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection^1-4: The biotinylated MQ2-39C3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MQ2-13A5 antibody (Cat. No. 5001102/501110) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization¹, Western blotting. The LEAF? purified MQ2-13A5 antibody (Endotoxin Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430501 to 430506) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
Gaines Das, R., et al. 1993. J. Immunol. Meth. 160:147.
Lesmeister, M. J., et al. 2005. Reproductive Biology and Endocrinology 3:74.

Product Citations

Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed

RRID

AB_2127615 (BioLegend Cat. No. 501201) AB_2249232 (BioLegend Cat. No. 501202)

Antigen Details

Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers, M., et al. 1993. Ann. Hematology 66:219.
Regulation: Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID: 3569 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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