Ultra-LEAF™ Purified anti-human IL-6 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2184	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7- expressed, recombinant human IL-6

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
CyTOF? - Verified
ELISPOT Capture, Neut, ICFC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 2.0 - 8.0 ?g/ml is recommended. For ELISA capture applications, a concentration range of 0.5 - 2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture^1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization^1-3,5,6: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry¹⁰.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
Zou JP, et al. 1999. J. Immunol. 162:4882.
Wyant TL, et al. 1999. Infect. Immun. 67:1338.
Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.

Product Citations

OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
Yang CY, et al. 2021. Stem Cell Res Ther. 193:12. PubMed

RRID

AB_2810625 (BioLegend Cat. No. 501125) AB_2810626 (BioLegend Cat. No. 501126)

Antigen Details

Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID: 3569 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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