Purified anti-human IL-6 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1692	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7- expressed, recombinant human IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

ELISA or ELISPOT Capture^1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization^1-3,5,6: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry¹⁰.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
Zou JP, et al. 1999. J. Immunol. 162:4882.
Wyant TL, et al. 1999. Infect. Immun. 67:1338.
Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.

Product Citations

Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
Neidleman J, et al. 2021. Elife. 10:. PubMed
Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
Schwabenland M, et al. 2021. Immunity. . PubMed
Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed

RRID

AB_2562841 (BioLegend Cat. No. 501115)

Antigen Details

Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID: 3569 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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