FITC anti-human IL-6 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1128	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7- expressed, recombinant human IL-6

Formulation

test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

?g sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)

Application Notes

ELISA or ELISPOT Capture^1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization^1-3,5,6: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry¹⁰.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
Zou JP, et al. 1999. J. Immunol. 162:4882.
Wyant TL, et al. 1999. Infect. Immun. 67:1338.
Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.

Product Citations

Peric Z, et al. 2015. Biol Blood Marrow Transplant. 21: 1431-1436. PubMed
Said A, et al. 2014. J Immunol. 193:6135. PubMed
Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
Ehrenstein M, et al. 2015. J Immunol. 194:2482. PubMed
Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed

RRID

AB_315151 (BioLegend Cat. No. 501103) AB_315153 (BioLegend Cat. No. 501105) AB_315152 (BioLegend Cat. No. 501104)

Antigen Details

Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID: 3569 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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