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PE anti-human IL-2 Antibody
产品名称：
PE anti-human IL-2 Antibody
产品类别：
抗体
产品编号：
500306
产品应用：
500306
[价格]
规格 价格 库存
25tests ￥ 1176 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 ?g/mL?is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 431807) is specially developed and recommended.

Clone?MQ1-17H12?cross-reacts to Cat15

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  3. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  4. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  5. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  6. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  7. Costa-Martins AG, et al. 2021. Cell Rep Med. 2:100465. PubMed
  8. Lindesmith LC, et al. 2020. Cell Mol Gastroenterol Hepatol. 0.586805556. PubMed
  9. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  10. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  11. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  12. van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
  13. Bartolomé-Casado R, et al. 2020. Mucosal Immunol. 14:402. PubMed
  14. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  15. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  16. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  17. Mikkelsen S, et al. 2010. Virology. 403:163. PubMed
  18. Gao X, et al. 2022. iScience. 25:104911. PubMed
  19. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  20. Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed
  21. Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed
  22. Dykema AG, et al. 2021. J Clin Invest. 131:. PubMed
  23. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  24. Sashihara J, et al. 2011. PLoS One. 6:e1002308. PubMed
  25. Shen C, et al. 2021. BMC Med. 19:283. PubMed
RRID
AB_315093 (BioLegend Cat. No. 500306) AB_315094 (BioLegend Cat. No. 500307)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

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