Brilliant Violet 605™ anti-human IL-2 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2904	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli - expressed recombinant human IL-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

ELISA or ELISPOT Capture^2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 ?g/mL?is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation², immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,4-6,8, neutralization¹³, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 431807) is specially developed and recommended.

Clone?MQ1-17H12?cross-reacts to Cat¹⁵

Application References (PubMed link indicates BioLegend citation)

Andersson J, et al. 1994. Immunology 83:16. (IHC)
Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
Wu Z, et al. 2015. J Virol. 89:6435. PubMed
Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed

Product Citations

Carre C, et al. 2021. iScience. 24:102970. PubMed
Seo YB, et al. 2021. Vaccines (Basel). 9: . PubMed
Thompson EA, et al. 2019. Cell Rep. 28:1127. PubMed
Ryan E, et al. 2016. PLoS Pathog. 12: 1005412. PubMed
Collins DR, et al. 2021. Immunity. 54:2372. PubMed
de Jonge K, et al. 2021. OncoImmunology. 10(1):1873585. PubMed
Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
Harper J, et al. 2021. Nat Commun. 12:2866. PubMed
Cimen Bozkus C, et al. 2021. STAR Protoc. 2:100758. PubMed

RRID

AB_11124349 (BioLegend Cat. No. 500331) AB_2563877 (BioLegend Cat. No. 500332)

Antigen Details

Structure: Cytokine; 15.4 kD (Mammalian)
Bioactivity: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources: T cells
Cell Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Regulation: Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID: 3558 View all products for this Gene ID
UniProt: View information about IL-2 on UniProt.org

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