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Alexa Fluor® 700 anti-human IL-2 Antibody
产品名称：
Alexa Fluor® 700 anti-human IL-2 Antibody
产品类别：
抗体
产品编号：
500319
产品应用：
500319
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The IL-2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 ?g/mL?is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 431807) is specially developed and recommended.

Clone?MQ1-17H12?cross-reacts to Cat15

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Masek-Hammerman K, et al. 2010. J Virol. 84:9810. PubMed
  2. Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
  3. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  4. Bailur JK, et al. 2020. JCI Insight. 5:00. PubMed
  5. Maksaereekul S, et al. 2009. Vaccine. 28:3754. PubMed
  6. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  7. Kassu A, et al. 2010. J Immunol. 185:3007. PubMed
  8. Vagenas P, et al. 2010. PLoS One. 5:e12891. PubMed
  9. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  10. Pillet S, et al. 2016. Clin Immunol. 168:72-87. PubMed
  11. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  12. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  13. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
RRID
AB_528928 (BioLegend Cat. No. 500319) AB_528929 (BioLegend Cat. No. 500320)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

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