Biotin anti-mouse IL-6 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7-expressed, recombinant mouse IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5 - 2 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL?are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1 - 4 ?g/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection^1-4,8 or ELISPOT Detection^5,6: The biotinylated MP5-32C11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified MP5-20F3 antibody (Cat. No. 504502/504506) as capture antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard.

Additional reported applications (for the relevant formats) include: Western blotting, In Vivo Capture⁷.

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Sander B, et al. 1993. J. Immunol. Meth. 166:201.
Starnes H, et al. 1990. J. Immunol. 145:4185.
Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181.
Mo X, et al. 1995. J. Virol. 69:1288.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
O'Connell PJ, et al. 2006. Blood 107:1010.

Product Citations

Lee J, et al. 2015. PLoS One. 10: 0142624. PubMed
Morita M, et al. 2021. Heliyon. 7:e06228. PubMed
Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed
Zhao L, et al. 2021. STAR Protocols. 2(3):100732. PubMed
Rosen DA, et al. 2019. Sci Transl Med. 11:eaau5266. PubMed
Chan M, et al. 2021. Mol Syst Biol. 17:e10426. PubMed

RRID

AB_2127458 (BioLegend Cat. No. 504601) AB_2233898 (BioLegend Cat. No. 504602)

Antigen Details

Structure: Neuropoietic cytokine family; 22-29 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID: 16193 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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