Ultra-LEAF™ Purified anti-mouse IL-6 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
50mg	￥ 28248	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7-expressed, recombinant mouse IL-6

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
CyTOF? - Verified
ELISPOT Capture, Neut, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL?are recommended for each ELISA plate. For ELISPOT applications, a concentration range of 4.0 - 8.0 ?g/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^1-3,11 or ELISPOT Capture^4,5: The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Neutralization^1-2,8-10: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. Nos. 504511-504516).

Additional reported applications (for relevant formats of this clone) include: In Vivo Capture⁷, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated⁶, and acetone-fixed frozen tissue sections^{13, 14}).

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. Nos. 431301 to 431306) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)
Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)
Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. (ELISPOT Capture)
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT Capture)
Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)
Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)
Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)
Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)
O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture)
Ozeki Y, et al. 2010. Int. Immunol. 22:179. PubMed
Galie M, et al. 2005. Carcinogenesis 26:1868 (IHC)
Schone MP, et al. 2000. J Immunol 165:6583 (IHC)
Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
Notley CA, et al. 2015. J Immunol. 194:1621. PubMed

Product Citations

Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed

RRID

AB_2814417 (BioLegend Cat. No. 504511) AB_2814418 (BioLegend Cat. No. 504512) AB_2814419 (BioLegend Cat. No. 504513) AB_2814420 (BioLegend Cat. No. 504514) AB_2814421 (BioLegend Cat. No. 504515) AB_2814422 (BioLegend Cat. No. 504516)

Antigen Details

Structure: Neuropoietic cytokine family; 22-29 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID: 16193 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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