Purified anti-human IL-4 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1692	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Pig, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO-expressed, recombinant human IL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization^1-3: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
Raqib R, et al. 1995. Infect. Immun. 63:289.
Andersson J, et al. 1994. Immunology 83:16.
Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
Kubota M, et al. 1997. J. Immunol. 158:5321.
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed

Product Citations

Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
Neidleman J, et al. 2021. Elife. 10:. PubMed
Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed

RRID

AB_2562840 (BioLegend Cat. No. 500829)

Antigen Details

Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of na?ve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565 View all products for this Gene ID
UniProt: View information about IL-4 on UniProt.org

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