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Brilliant Violet 605™ anti-human IL-4 Antibo
产品名称：
Brilliant Violet 605™ anti-human IL-4 Antibo
产品类别：
抗体
产品编号：
500827
产品应用：
500827
[价格]
规格 价格 库存
25tests ￥ 2712 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant human IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization1-3: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
  2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
  4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
  6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
  7. Raqib R, et al. 1995. Infect. Immun. 63:289.
  8. Andersson J, et al. 1994. Immunology 83:16.
  9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  10. Kubota M, et al. 1997. J. Immunol. 158:5321.
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed
Product Citations
  1. Arlehamn C, et al. 2016. PLoS Pathog. 12: 1005760. PubMed
  2. Bacher P et al. 2019. Cell. 176(6):1340-1355 . PubMed
  3. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  4. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  5. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
RRID
AB_2562311 (BioLegend Cat. No. 500827) AB_2563879 (BioLegend Cat. No. 500828)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of na?ve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

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