Biotin anti-human IL-4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Pig, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO-expressed, recombinant human IL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection, ICFC

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-4 protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate.

Application Notes

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization^1-3: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References (PubMed link indicates BioLegend citation)

Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
Raqib R, et al. 1995. Infect. Immun. 63:289.
Andersson J, et al. 1994. Immunology 83:16.
Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
Kubota M, et al. 1997. J. Immunol. 158:5321.
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed

Product Citations

Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
Kurtaj A, et al. 2017. J Allergy Clin Immunol. 140:1746. PubMed

RRID

AB_315122 (BioLegend Cat. No. 500803) AB_315123 (BioLegend Cat. No. 500804)

Antigen Details

Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of na?ve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565 View all products for this Gene ID
UniProt: View information about IL-4 on UniProt.org

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