Alexa Fluor® 488 anti-human IL-4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3180	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Pig, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO-expressed, recombinant human IL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization^1-3: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References (PubMed link indicates BioLegend citation)

Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
Raqib R, et al. 1995. Infect. Immun. 63:289.
Andersson J, et al. 1994. Immunology 83:16.
Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
Kubota M, et al. 1997. J. Immunol. 158:5321.
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed

Product Citations

Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed

RRID

AB_493324 (BioLegend Cat. No. 500817)

Antigen Details

Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of na?ve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565 View all products for this Gene ID
UniProt: View information about IL-4 on UniProt.org

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