PE anti-mouse IL-3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant mouse IL-3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA or ELISPOT Capture^1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Abrams, J., et al. 1988. J. Immunol. 140:131.
Cockayne, D., et al. 1991. Growth Factors 5:171.
Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Finkelman, F., et al. 1993. J. Immunol. 151:1235.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Karulin, A., et al. 2000. J. Immunol. 164:1862.

Product Citations

Price J, et al. 2015. Diabetes. 64: 3521 - 3531. PubMed

RRID

AB_315309 (BioLegend Cat. No. 503903) AB_315310 (BioLegend Cat. No. 503904)

Antigen Details

Structure: Cytokine; 28 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID: 16187 View all products for this Gene ID
UniProt: View information about IL-3 on UniProt.org

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