Biotin anti-mouse IL-3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3936	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-expressed, recombinant mouse IL-3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate.
For use as an ELISPOT detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection^1-5,7: The biotinylated MP2-43D11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP2-8F8 antibody (Cat. No. 503902/503906) as the capture antibody.
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting, and immunohistochemical staining⁶ of paraformaldehyde-fixed, saponin-treated tissue sections.

Application References (PubMed link indicates BioLegend citation)

Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Abrams, J., et al. 1988. J. Immunol. 140:131.
Cockayne, D., et al. 1991. Growth Factors 5:171.
Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Sander, B., et al. 1991. Immunol. Rev. 119:65.
Karulin, A., et al. 2000. J. Immunol. 164:1862.

RRID

AB_2123875 (BioLegend Cat. No. 504002)

Antigen Details

Structure: Cytokine; 28 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID: 16187 View all products for this Gene ID
UniProt: View information about IL-3 on UniProt.org

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