Purified anti-mouse GM-CSF Antibody
- 产品名称：
- Purified anti-mouse GM-CSF Antibody
- 产品类别：
- 抗体
- 产品编号：
- 505503
- 产品应用：
- 505503
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Yeast-expressed, recombinant mouse GM-CSF.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, the antibody should be titrated between 1-4 ?g/ml to determine optimal concentration. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Detection1,3-5: The biotinylated MP1-31G6 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP1-22E9 antibody (Cat. No. 505402/505408) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation4, and Neutralization2-4. The LEAF? purified MP1-22E9 antibody (Endotoxin in vivo and in vitro (Cat. No. 505408).
- Application References
(PubMed link indicates BioLegend citation) -
- Sander B, et al. 1993. J. Immunol. Meth. 166:201.
- Suda T, et al. 1990. Cell. Immunol. 129:228.
- Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
- Uyttenhove C, et al. 2011. J Leukoc Biol. 89:1001. PubMed.
- Product Citations
-
- Uyttenhove C, et al. 2011. J Leukoc Biol. 89:1001. PubMed
- RRID
- AB_2085380 (BioLegend Cat. No. 505503) AB_2085379 (BioLegend Cat. No. 505504)
- Structure
- Cytokine; 22 kD (Mammalian)
- Bioactivity
- Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
- Cell Sources
- T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
- Cell Targets
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
- Receptors
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
- Regulation
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Gene ID
- 12981 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org
