Biotin anti-mouse GM-CSF Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant mouse GM-CSF.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection^1,3-5: The biotinylated MP1-31G6 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP1-22E9 antibody (Cat. No. 505402/505408) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation⁴, and Neutralization^2-4. The LEAF? purified MP1-22E9 antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse GM-CSF bioactivity in vivo and in vitro (Cat. No. 505408).

Application References (PubMed link indicates BioLegend citation)

Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
Suda, T., et al. 1990. Cell. Immunol. 129:228.
Nozaki, S., et al. 1991. J. Invest. Dermatol. 97:10.
Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.

Product Citations

Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
Chan M, et al. 2021. Mol Syst Biol. 17:e10426. PubMed

RRID

AB_315385 (BioLegend Cat. No. 505501) AB_2085381 (BioLegend Cat. No. 505502)

Antigen Details

Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 12981 View all products for this Gene ID
UniProt: View information about GM-CSF on UniProt.org

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