Purified anti-mouse GM-CSF Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 996	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant mouse GM-CSF

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, ICC, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, the antibody should be titrated between 1-4 ?g/ml to determine optimal concentration. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture^1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.
Flow Cytometry⁸: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization^2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include:?immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,6,7, and immunocytochemistry⁸.

Application References (PubMed link indicates BioLegend citation)

Sander B, et al. 1993. J. Immunol. Methods 166:201.
Suda T, et al. 1990. Cell. Immunol. 129:228.
Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
Abrams JS, et al. 1992. Immunol. Rev. 127:5.
Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
Sander B, et al. 1991. Immunol. Rev. 119:65.
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Larkin J, et al. 2006. J. Immunol. 177:268.

Product Citations

Wu C, et al. 2020. J Immunol. 204:212. PubMed
Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
Mei S, et al. 2015. PLoS One. 10: 0135867. PubMed

RRID

AB_315377 (BioLegend Cat. No. 505401) AB_315378 (BioLegend Cat. No. 505402)

Antigen Details

Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 12981 View all products for this Gene ID
UniProt: View information about GM-CSF on UniProt.org

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