Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

ELISA or ELISPOT Capture^1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.
Flow Cytometry⁸: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization^2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include:?immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,6,7, and immunocytochemistry⁸.

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