PE anti-mouse IFN-γR β chain Antibody
- 产品名称：
- PE anti-mouse IFN-γR β chain Antibody
- 产品类别：
- 抗体
- 产品编号：
- 113603
- 产品应用：
- 113603
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse IFN-γR β chain protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1?and immunoprecipitation1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113604) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Bach EA,?et al.?1995.?Science?270:1215.
- Product Citations
-
- Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
- Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
- Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
- Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
- Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
- Shou P, et al. 2016. Oncogene. 10.1038/onc.2016.128. PubMed
- RRID
- AB_313560 (BioLegend Cat. No. 113603) AB_313561 (BioLegend Cat. No. 113604)
- Structure
- Class II cytokine receptor family, associates with CDw119, 60-65 kD, AF-1
- Distribution
-
T and B cells, NK cells, monocytes, macrophages, granulocytes, fibroblasts
- Function
- Regulates immune response, upregulates MHC class I expression
- Ligand/Receptor
- IFN-γ
- Cell Type
- B cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Bach EA,?et al.?1995.?Science?270:1215.?
2. Ansel KM,?et al.?2003.?Nat. Immunol.?4:616.
- Gene ID
- 15978 View all products for this Gene ID
- UniProt
- View information about IFN-gamma R beta chain on UniProt.org
