PE anti-Notch 1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1248	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Notch1 GST fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications of this antibody (for the relevant formats) include: Western blotting^1,2,3, and immunohistochemistry^4,5.

Application References (PubMed link indicates BioLegend citation)

Huppert SS, et al. 2000. Nature 405:966. (WB)
Ray WJ, et al. 1999. Proc. Natl. Acad. Sci. USA 96:3263. (WB)
DeStrooper B, et al. 1999. Nature 398:518. (WB)
Sun H, et al. 2007. J. Cell Biol. 177:647. (IHC) PubMed
Jundt F, et al. 2008. Leukemia. 22:1587. (IHC) PubMed
Liu JC, et al. 2012. PNAS 109:5832. PubMed.

Product Citations

Britton GJ, et al. 2017. Elife. 6:. PubMed
Kongkavitoon P, et al. 2016. PLoS One. 11: 0146696. PubMed

RRID

AB_2282905 (BioLegend Cat. No. 629105) AB_2251468 (BioLegend Cat. No. 629106)

Antigen Details

Structure: Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 36 EGF repeats, 3 Notch/Lin repeats. Predicted molecular weight 271 kD.
Distribution: Highly expressed in the brain, lung, and thymus (CD4^-CD8^- cells and CD4^-CD8⁺ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart.
Function: Receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions.
Modification: Phosphorylation
Cell Type: T cells, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Huppert SS, et al. 2000. Nature 405:966.
2. Kopan R, et al. 1993. J. Cell Biol. 121:631.
3. Saxena MT, et al. 2001. J. Biol. Chem. 276:40268.
4. Mizutani T, et al. 2001. Proc. Natl. Acad. Sci. USA 98:9026.
Gene ID: 4851 View all products for this Gene ID
UniProt: View information about Notch1 on UniProt.org

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