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Purified anti-human Mesothelin Antibody
产品名称:
Purified anti-human Mesothelin Antibody
产品类别:
抗体
产品编号:
530101
产品应用:
530101
[价格]
规格 价格 库存
50µg ¥ 1764 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DNA immunization (mesothelin expression plasmid) with mesothelin-Fc fusion protein for final boost.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

FC - Verified
ELISA Detection, FC, IHC-P, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2 ?g/ml of the capture antibody was utilized to generate the example standard curve for human mesothelin. For flow cytometric staining, the suggested use of this reagent is 0.25 - 1.0 ?g per million cells in 100 ?L volume. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: To measure human mesothelin, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated (clone MB) antibody (Cat. No. 520203) as the detection antibody. Recombinant Human Mesothelin (ELISA Std.) (Cat. No. 593209) can be used as the protein standard.

Note: For testing human mesothelin in serum, plasma, or supernatant, LEGEND MAX? Human Mesothelin ELISA Kit (Cat. No. 438607 & 438608) is specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Onda M, et al. 2005. Clin. Cancer Res. 11:5840.
RRID
AB_2571908 (BioLegend Cat. No. 530101)

Antigen Details

Structure
~40 kD glycoprotein that can be membrane-bound, or relased from the membrane in a soluble form.
Distribution

Mesothelial cells lining the pleura, peritoneum, and pericardium. It is also located in various cancer cells.

Function
Not known. May promote carcinogenesis and metastasis.
Interaction
Various cancer cells such as ovarian and pancreatic cancer cells.
Ligand/Receptor
CA125 (MUC16).
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pastan I, et al. 2014. Cancer Res. 74:2907.
2. Hellstrom I, et al. 2011. Expert Opin. Med. Diagn. 1:227.
3. Hassan R, et al. 2008. Eur. J. Cancer 44:46.
4. Hassan R, et al. 2006. Clin. Cancer Res. 2:447.
5. Onda M, et al. 2005. Clin. Cancer Res. 16:5840-6.
6. Hassan R, et al. 2004. Clin. Cancer Res. 10:3937.
7. Scholler N, et al. 1999. Proc. Natl. Acad. Sci. 96:11531.

Gene ID
10232 View all products for this Gene ID
UniProt
View information about Mesothelin on UniProt.org

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