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Purified anti-mouse IL-27 p28 Antibody
产品名称:
Purified anti-mouse IL-27 p28 Antibody
产品类别:
抗体
产品编号:
516901
产品应用:
516901
[价格]
规格 价格 库存
50µg ¥ 1344 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-27-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by?ELISA assay. A concentration of 0.5 ?g/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1 We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.

ELISA Capture:?To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6?antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No.?577409) can be used as the protein standard.
Flow Cytometry:?The fluorochrome-labeled MM27-7B1?antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note:?For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX? Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707?& 438708) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Uyttenhove C, et al. 2011. J. Leuko. Biol. 89:1001.
RRID
AB_2561334 (BioLegend Cat. No. 516901) AB_2561335 (BioLegend Cat. No. 516902)

Antigen Details

Structure
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution

Produced by antigen-presenting cells, including macrophages and dendritic cells.

Function
Drives rapid clonal expansion of na?ve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction
T cells and NK cells.
Ligand/Receptor
IL-27R (TCCR/WSX-1).
Cell Type
Antigen-presenting cells, Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Salcedo R,?et al.?2004.?J. Immunol.?173:7170.
2. Owaki T,?et al.?2005.?J. Immunol.?175:2191.
3. Pflanz S,?et al.?2002.?Immunity?16:779.
4. Diveu C,?et al.?2009.?J. Immunol.?182:5748.
5. Morishima N,?et al.?2005.?J. Immunol.?175:1686.
6. Liu J,?et al.?2007.?J. Exp. Med.?204:141.

Gene ID
246779 View all products for this Gene ID
UniProt
View information about IL-27 on UniProt.org

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