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PE anti-mouse IL-27 p28 Antibody
产品名称:
PE anti-mouse IL-27 p28 Antibody
产品类别:
抗体
产品编号:
516908
产品应用:
516908
[价格]
规格 价格 库存
100µg ¥ 4020 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-27-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1 We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.

ELISA Capture:?To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6?antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No.?577409) can be used as the protein standard.
Flow Cytometry:?The fluorochrome-labeled MM27-7B1?antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note:?For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX? Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707?& 438708) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Uyttenhove C, et al. 2011. J. Leuko. Biol. 89:1001.
Product Citations
  1. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  2. Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed
RRID
AB_10642965 (BioLegend Cat. No. 516908)

Antigen Details

Structure
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution

Produced by antigen-presenting cells, including macrophages and dendritic cells.

Function
Drives rapid clonal expansion of na?ve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction
T cells and NK cells.
Ligand/Receptor
IL-27R (TCCR/WSX-1).
Cell Type
Antigen-presenting cells, Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Salcedo R,?et al.?2004.?J. Immunol.?173:7170.
2. Owaki T,?et al.?2005.?J. Immunol.?175:2191.
3. Pflanz S,?et al.?2002.?Immunity?16:779.
4. Diveu C,?et al.?2009.?J. Immunol.?182:5748.
5. Morishima N,?et al.?2005.?J. Immunol.?175:1686.
6. Liu J,?et al.?2007.?J. Exp. Med.?204:141.

Gene ID
246779 View all products for this Gene ID
UniProt
View information about IL-27 on UniProt.org

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