| 规格 | 价格 | 库存 |
|---|---|---|
| 100µg | ¥ 4056 | 1 |
ICFC - Quality tested
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1 We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.
ELISA Capture:?To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6?antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No.?577409) can be used as the protein standard.
Flow Cytometry:?The fluorochrome-labeled MM27-7B1?antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note:?For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX? Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707?& 438708) are specially developed and recommended.
Produced by antigen-presenting cells, including macrophages and dendritic cells.
1. Salcedo R,?et al.?2004.?J. Immunol.?173:7170.
2. Owaki T,?et al.?2005.?J. Immunol.?175:2191.
3. Pflanz S,?et al.?2002.?Immunity?16:779.
4. Diveu C,?et al.?2009.?J. Immunol.?182:5748.
5. Morishima N,?et al.?2005.?J. Immunol.?175:1686.
6. Liu J,?et al.?2007.?J. Exp. Med.?204:141.