Alexa Fluor® 647 anti-mouse IL-27 p28 Antibody-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4164	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Mouse IL-27-OVA

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.¹We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.

ELISA Capture:?To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6?antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No.?577409) can be used as the protein standard.
Flow Cytometry:?The fluorochrome-labeled MM27-7B1?antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note:?For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX? Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707?& 438708) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Uyttenhove C, et al. 2011. J. Leuko. Biol. 89:1001.

RRID

AB_2125060 (BioLegend Cat. No. 516904)

Antigen Details

Structure: A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution: Produced by antigen-presenting cells, including macrophages and dendritic cells.
Function: Drives rapid clonal expansion of na?ve CD4⁺ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction: T cells and NK cells.
Ligand/Receptor: IL-27R (TCCR/WSX-1).
Cell Type: Antigen-presenting cells, Dendritic cells, Macrophages
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Salcedo R,?et al.?2004.?J. Immunol.?173:7170.
2. Owaki T,?et al.?2005.?J. Immunol.?175:2191.
3. Pflanz S,?et al.?2002.?Immunity?16:779.
4. Diveu C,?et al.?2009.?J. Immunol.?182:5748.
5. Morishima N,?et al.?2005.?J. Immunol.?175:1686.
6. Liu J,?et al.?2007.?J. Exp. Med.?204:141.
Gene ID: 246779 View all products for this Gene ID
UniProt: View information about IL-27 on UniProt.org

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