PE anti-mouse CD274 (B7-H1, PD-L1) Antibody
- 产品名称：
- PE anti-mouse CD274 (B7-H1, PD-L1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 155403
- 产品应用：
- 155403
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-L1 transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The antibody MIH7 does not block other CD274 antibodies: clone MIH6 and 10F.9G2.
Additional reported applications (for the relevant formats) include: Blocking (Block)
- Application References
(PubMed link indicates BioLegend citation) -
- Sharma MD, et al. 2007. J Clin Invest. 117:2570-82. (Block)
- Sharma MD, et al. 2015. Sci Adv. 1:e1500845. (Block)
- Product Citations
-
- Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
- Kalbasi A, et al. 2020. Sci Transl Med. :12. PubMed
- Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
- Wagner AK, et al. 2022. iScience. 25:105137. PubMed
- Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
- RRID
- AB_2728222 (BioLegend Cat. No. 155403) AB_2728223 (BioLegend Cat. No. 155404)
- Structure
- Type 1 transmembrane protein, member of the B7 family, 40kD.
- Distribution
-
T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (CD279)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Monocytes, T cells
- Biology Area
- Cancer Biomarkers, Immuno-Oncology, Immunology, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Dorand RD. 2016. Science. 353:399
- Khan AR, et al. 2015. Nat Commun. 6:5997
- Kiyasu J, et al. 2015. Blood. 126:2193
- Herold M, et al. 2015 J Immunol. 195:3584
- Buddhisa S, et al. 2015. J Immunol. 194:4413
- Gene ID
- 60533 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
