Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1
- 产品类别：
- 抗体
- 产品编号：
- 153602
- 产品应用：
- 153602
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-L1-transfected cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC -?Quality tested
Blocking -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
mAb MIH6 blocks the binding of mouse PD-L1 to PD-1 (CD279)
- Application References
(PubMed link indicates BioLegend citation) -
- Gassner FJ, et al. 2015. Br J Haematol. 170:515 (Block)
- Haile ST, et al. 2013. J Immunol. 191:2829 (FC)
- Hirahara K, et al. 2012. Immunity. 36:1017 (Block)
- Fife BT, et al. 2009. Nat Immunol. 10:1185 (Block)
- Kanai T, et al. 2003. J Immunol. 171:4156 (Block)
- Product Citations
-
- Blomberg E, et al. 2022. Neurooncol Adv. 4:vdac148. PubMed
- RRID
- AB_2715965 (BioLegend Cat. No. 153602) AB_2715966 (BioLegend Cat. No. 153603)
- Structure
- Type 1 transmembrane protein, member of the B7 family, 40kD.
- Distribution
-
T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (CD279)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
- Biology Area
- Cancer Biomarkers, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Dorand RD. 2016.?Science. 353:399.
2.?Khan AR,?et al. 2015.?Nat Commun. 6:5997.
3.?Kiyasu J, et al. 2015.?Blood. 126:2193
4.?Herold M, et al. 2015.?J Immunol. 195:3584
5.?Buddhisa S, et? al. 2015.?J Immunol. 194:4413
- Gene ID
- 60533 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
