TotalSeq™-A0190 anti-mouse CD274 (B7-H1, PD--上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse PD-L1-transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-A oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

mAb MIH6 blocks the binding of mouse PD-L1 to PD-1 (CD279)

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our?Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Gassner FJ, et al. 2015. Br J Haematol. 170:515 (Block)
Haile ST, et al. 2013. J Immunol. 191:2829 (FC)
Hirahara K, et al. 2012. Immunity. 36:1017 (Block)
Fife BT, et al. 2009. Nat Immunol. 10:1185 (Block)
Kanai T, et al. 2003. J Immunol. 171:4156 (Block)

Product Citations

Golomb SM, et al. 2020. Cell Rep. 33:108438. PubMed
Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
Guldner IH, et al. 2021. STAR Protocols. 2(2):100537. PubMed

RRID

AB_2783125 (BioLegend Cat. No. 153604)

Antigen Details

Structure: Type 1 transmembrane protein, member of the B7 family, 40kD.
Distribution: T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Function: CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor: PD-1 (CD279)
Cell Type: B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Dorand RD. 2016.?Science. 353:399.
2.?Khan AR,?et al. 2015.?Nat Commun. 6:5997.
3.?Kiyasu J, et al. 2015.?Blood. 126:2193
4.?Herold M, et al. 2015.?J Immunol. 195:3584
5.?Buddhisa S, et? al. 2015.?J Immunol. 194:4413
Gene ID: 60533 View all products for this Gene ID
UniProt: View information about CD274 on UniProt.org

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