Ultra-LEAF™ Purified anti-human CD274 (B7-H1
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD274 (B7-H1
- 产品类别：
- 抗体
- 产品编号：
- 374502
- 产品应用：
- 374502
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PD-L1-transfected cells.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include blocking1, 2.
Clone MIH3 blocks the binding of PD-L1 with PD-1 , but did not block the binding of PD-L1 with B7-1 (CD80).
- Application References
(PubMed link indicates BioLegend citation) -
- Haile S.T,?et al. 2014.?Cancer Immunol Res. 2:1. (Block)
- Butte M.J, et al. 2008. Mol Immunol. 45:3567. (Block)
- RRID
- AB_2715967 (BioLegend Cat. No. 374502) AB_2715968 (BioLegend Cat. No. 374503)
- Structure
- Type 1 transmembrane protein, member of the B7 family, 40kD.
- Distribution
-
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (CD279), B7-1 (CD80).
- Cell Type
- T cells, B cells, NK cells, Monocytes, Macrophages, Granulocytes, Dendritic cells
- Biology Area
- Immunology, Cancer Biomarkers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Khan AR,?et al. 2015.?Nat Commun. 6:5997.
2. Kiyasu J, et al. 2015. Blood. 126:2193.
3. Herold M, et al. 2015 J Immunol. 195:3584.
4. Buddhisa S, et al. 2015. J Immunol. 194:4413.
- Gene ID
- 29126 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
