PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) Antibo
- 产品名称：
- PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 374505
- 产品应用：
- 374505
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PD-L1-transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include blocking1, 2.
Clone MIH3 blocks the binding of PD-L1 with PD-1 , but did not block the binding of PD-L1 with B7-1 (CD80).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Haile S.T,?et al. 2014.?Cancer Immunol Res. 2:1. (Block)
- Butte M.J, et al. 2008. Mol Immunol. 45:3567. (Block)
- Product Citations
-
- Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
- Ma S, et al. 2022. Nat Commun. 13:4118. PubMed
- RRID
- AB_2734433 (BioLegend Cat. No. 374505) AB_2734434 (BioLegend Cat. No. 374506)
- Structure
- Type 1 transmembrane protein, member of the B7 family, 40kD.
- Distribution
-
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (CD279), B7-1 (CD80).
- Cell Type
- T cells, B cells, NK cells, Monocytes, Macrophages, Granulocytes, Dendritic cells
- Biology Area
- Immunology, Cancer Biomarkers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Khan AR,?et al. 2015.?Nat Commun. 6:5997.
2. Kiyasu J, et al. 2015. Blood. 126:2193.
3. Herold M, et al. 2015 J Immunol. 195:3584.
4. Buddhisa S, et al. 2015. J Immunol. 194:4413.
- Gene ID
- 29126 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
