APC/Fire™ 810 anti-human CD274 (B7-H1,PD-L1)
- 产品名称：
- APC/Fire™ 810 anti-human CD274 (B7-H1,PD-L1)
- 产品类别：
- 抗体
- 产品编号：
- 374515
- 产品应用：
- 374515
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PD-L1-transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire? 810 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire? 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include blocking1, 2.
Clone MIH3 blocks the binding of PD-L1 with PD-1 , but did not block the binding of PD-L1 with B7-1 (CD80).
- Application References
(PubMed link indicates BioLegend citation) -
- Haile S.T,?et al. 2014.?Cancer Immunol Res. 2:1. (Block)
- Butte M.J, et al. 2008. Mol Immunol. 45:3567. (Block)
- RRID
- AB_2927810 (BioLegend Cat. No. 374515)
- Structure
- Type 1 transmembrane protein, member of the B7 family, 40kD.
- Distribution
-
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (CD279), B7-1 (CD80).
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Cancer Biomarkers, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Khan AR,?et al. 2015.?Nat Commun. 6:5997.
- Kiyasu J, et al. 2015. Blood. 126:2193-201.
- Herold M, et al. 2015. J Immunol. 195:3584-95.
- Buddhisa S, et al. 2015. J Immunol. 194:4413-21.
- Gene ID
- 29126 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
