Purified anti-human CD272 (BTLA) Antibody
- 产品名称:
- Purified anti-human CD272 (BTLA) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 344502
- 产品应用:
- 344502
- Verified Reactivity
- Human
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BTLA transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Agonist - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of T cell proliferation and cytokine production1. Clone MIH26 has agonistic activity on BTLA, resulting in the inhibition of activation.
The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for use in in vitro and in vivo biofunctional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121.
- Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.
- Product Citations
-
- Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
- Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
- Wiedemann A, et al. 2021. Front Immunol. 12:667991. PubMed
- Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
- RRID
- AB_2043950 (BioLegend Cat. No. 344502)
- Structure
- Ig superfamily, with similar structure to CTLA-4 and PD-1
- Distribution
-
B cells and T lymphocytes, NK cells and dendritic cells
- Function
- Negative regulation of T cell activation, proliferation and cytokine production
- Ligand/Receptor
- HVEM
- Cell Type
- B cells, Dendritic cells, NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
2. Sun Y, et al. 2009. J. Immunol. 183:1946.
3. Gonzalez LC, et al. 2005. P. Natl. Acad. Sci. USA 102:1116.
- Gene ID
- 151888 View all products for this Gene ID
- UniProt
- View information about CD272 on UniProt.org