Ultra-LEAF™ Purified anti-human CD273 (B7-DC
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD273 (B7-DC
- 产品类别：
- 抗体
- 产品编号：
- 345521
- 产品应用：
- 345521
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human B7-DC transfected cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, IHC-P, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 345504).
- Application References
(PubMed link indicates BioLegend citation) -
- Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
- Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
- Hobo W, et al. 2010. Blood 25:4501. (FC)
- Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
- Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
- Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
- RRID
- AB_2783235 (BioLegend Cat. No. 345521) AB_2783236 (BioLegend Cat. No. 345522)
- Structure
- B7 Immunoglobulin superfamily, 25 kD
- Distribution
-
Dendritic cells, liver, few transformed cell lines, subset of macrophages
- Function
- Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
- Ligand/Receptor
- PD-1
- Cell Type
- Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.
- Gene ID
- 80380 View all products for this Gene ID
- UniProt
- View information about CD273 on UniProt.org
