Purified anti-human CD273 (B7-DC, PD-L2) Antibody
- 产品名称：
- Purified anti-human CD273 (B7-DC, PD-L2) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 345502
- 产品应用：
- 345502
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human B7-DC transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IHC-P, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 345504).
- Application References
(PubMed link indicates BioLegend citation) -
- Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
- Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
- Hobo W, et al. 2010. Blood 25:4501. (FC)
- Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
- Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
- Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
- Product Citations
-
- Baram T, et al. 2022. Cancers (Basel). 14:. PubMed
- Muthumani K, et al. 2012. J Immunol. 187:2932. PubMed
- Kim MH, et al. 2018. Cancer Immunol Res. 0.427083333. PubMed
- Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
- RRID
- AB_1953319 (BioLegend Cat. No. 345502)
- Structure
- B7 Immunoglobulin superfamily, 25 kD
- Distribution
-
Dendritic cells, liver, few transformed cell lines, subset of macrophages
- Function
- Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
- Ligand/Receptor
- PD-1
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.
- Gene ID
- 80380 View all products for this Gene ID
- UniProt
- View information about CD273 on UniProt.org
