Alexa Fluor® 647 anti-mouse CD83 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD83 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 121514
- 产品应用：
- 121514
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD83 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Cramer SO, et al. 2000. Int. Immunol. 12:1347.
- Fujimoto Y, et al. 2002. Cell 108:755.
- Mott KR, et al. 2009. Virol J. 6:56. (FC) PubMed
- Roland Cl, et al. 2009. Mol Cancer Res. 8:1761. (IHC) PubMed
- Masuda Y, et al. 2010. Cancer Immunol Immunother. [Epub ahead of print] (FC) PubMed
- Tze LE, et al. 2011. J Exp Med. PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
-
- Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
- RRID
- AB_2074746 (BioLegend Cat. No. 121514)
- Structure
- Type I transmembrane protein, Ig superfamily, 45kD
- Distribution
-
Mature dendritic cells, activated lymphocytes
- Function
- Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
- Ligand/Receptor
- CD83 ligand
- Cell Type
- Dendritic cells, Lymphocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132.
2. Kotxor N, et al. 2004. Immunobiology 209:129.
3. Leon F, et al. 2004. J. Immunol. 173:2995.
4. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
5. Fujimoto Y, et al. 2002. Cell 108:755.
- Gene ID
- 12522 View all products for this Gene ID
- UniProt
- View information about CD83 on UniProt.org
