Ultra-LEAF™ Purified anti-mouse CD47 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD47 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 127519
- 产品应用：
- 127519
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking2?and immunohistochemical staining of frozen sections3. This clone is not cross-reactive with human CD47.
- Application References
(PubMed link indicates BioLegend citation) -
- Wang H,?et al.?2007.?P. Natl. Acad. Sci. USA?104:13744.
- Jiang P,?et al.?1999?J. Biol. Chem.?274:559. (IHC-F, Block)
- Product Citations
-
- Zhou Y, et al. 2022. Theranostics. 12:5488. PubMed
- Morrissey MA, et al. 2020. Immunity. 53(2):290-302.e6. PubMed
- RRID
- AB_2810387 (BioLegend Cat. No. 127520) AB_2571996 (BioLegend Cat. No. 127517) AB_2571997 (BioLegend Cat. No. 127518) AB_2571998 (BioLegend Cat. No. 127519) AB_2810388 (BioLegend Cat. No. 127533) AB_2810389 (BioLegend Cat. No. 127534)
- Structure
- 50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
- Distribution
-
Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
- Function
- Adhesion molecules, immune regulation.
- Interaction
- Associates with β3 integrins.
- Ligand/Receptor
- SIRPα (CD172α, Signal regulatory protein alpha), thrombospondin.
- Bioactivity
- Upon interaction with SIRPα, controls innate immune effector function, such as host cell phagocytosis.
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
2. Mawby WJ, et al. 1994. Biochem. J. 304:525.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Oldenborg PA, et al. 2000. Science 288:2051.
5. van Beek EM, et al. 2005. J. Immunol. 175:7781.
- Gene ID
- 16423 View all products for this Gene ID
- UniProt
- View information about CD47 on UniProt.org
