PE anti-human Notch 1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1404	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human Notch1-Fc fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking Notch 1 mediated binding to DLL4 in human cord blood CD34⁺ cells¹.

Application References (PubMed link indicates BioLegend citation)

Haraguchi K, et al. 2009. J. Immunol. 182:6168. (Block)
Yamanda S, et al. 2009. Blood 113:3631. (FC)
Guy CS. et al. 2013. Nat Immunol. 14:262. PubMed

Product Citations

Kumar V, et al. 2022. Cells. 11:. PubMed
Pozzo F, et al. 2017. Leukemia. 10.1038/leu.2017.90. PubMed
Canté-Barrett K, et al. 2022. JCI Insight. :. PubMed

RRID

AB_10896419 (BioLegend Cat. No. 352105) AB_10900817 (BioLegend Cat. No. 352106)

Antigen Details

Structure: Transmembrane protein. The extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 CDC10/ANK, 1 PEST motif, and 1 RAM23 domain.
Distribution: Highly expressed in the brain, lung, and thymus. Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Function: Regulates development, differentiation, and survival of a broad spectrum of cell lineages. Involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Interaction: RBP-J κ.
Ligand/Receptor: Jagged 1, Jagged 2, Delta 1, Delta 4.
Cell Type: B cells, Neural Stem Cells, Thymocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Vicente R, et al. 2010. Semin. Immunol. 22:270.
2. Zhao WL. 2010. Leukemia 24:13.
3. Sanda T, et al. 2010. Blood 115:1735.
4. Zhou J, et al. 2009. Immunity 31:356.
Gene ID: 4851 View all products for this Gene ID
UniProt: View information about Notch 1 on UniProt.org

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