Purified anti-human IL-9 Antibody
- 产品名称：
- Purified anti-human IL-9 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 507602
- 产品应用：
- 507602
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Baculovirus-expressed, recombinant human IL-9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 1000 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF? purified MH9D1 antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-9 bioactivity.
- Application References
(PubMed link indicates BioLegend citation) -
- Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528.
- Faulkner H, et al. 2002. J. Infec. Diseas. 185:665.
- Chen J, et al. 2008. Blood 111:5163. PubMed
- Chang HC, et al. 2010. Nat. Immunol. 11:527. (ELISA) PubMed
- Lozano E, et al. 2012. J Immunol. 188:3869. PubMed.
- Product Citations
-
- Chen J, et al. 2008. Blood. 111:5163. PubMed
- Lozano E, et al. 2012. J Immunol. 188:3678. PubMed
- RRID
- AB_315484 (BioLegend Cat. No. 507602)
- Structure
- Cytokine; 32-39 kD (Mammalian)
- Bioactivity
- Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
- Cell Sources
- IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
- Cell Targets
- T lymphocytes, erythroid precursors
- Receptors
- IL-9R
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.
- Regulation
- Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
- Gene ID
- 3578 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
