Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA
- 产品类别：
- 抗体
- 产品编号：
- 143723
- 产品应用：
- 143723
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- PD-1H- IgG Fc fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Flies DB, et al. 2011. J. Immunol. 187:1537.
- RRID
- AB_2783107 (BioLegend Cat. No. 143723) AB_2783108 (BioLegend Cat. No. 143724)
- Structure
- Type I transmembrane protein, member of the CD28 family
- Distribution
-
Macrophages, dendritic cells, neutrophils, NK cells, na?ve and activated T cells
- Function
- Negative regulator of the immune response
- Cell Type
- Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Antigen References
-
1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.
- Gene ID
- 74048 View all products for this Gene ID
- UniProt
- View information about PD-1H on UniProt.org
