Ultra-LEAF™ Purified anti-mouse/rat CD178 (F
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse/rat CD178 (F
- 产品类别：
- 抗体
- 产品编号：
- 106707
- 产品应用：
- 106707
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro and in vivo FasL blockade1-4. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Trinité B, et al. 2000. J. Immunol. 165:4202. (Block)
- Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914. (Block)
- Chang CL, et al. 2007. J. Immunol. 178:6984. (Block) PubMed
- Borges VM, et al. 2001. J. Exp. Med. 194:155. (Block)
- Product Citations
-
- Koda Y, et al. 2021. Nat Commun. 12:4474. PubMed
- RRID
- AB_2810345 (BioLegend Cat. No. 106707) AB_2810346 (BioLegend Cat. No. 106708)
- Structure
- TNF superfamily, 40 kD
- Distribution
-
Activated T cells, spleen, testis, eye
- Function
- Induces apoptosis
- Ligand/Receptor
- Fas (CD95)
- Cell Type
- T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Nagata S. 1999. Annu. Rev. Genet. 33:209.
3. Takahashi T, et al. 1994. Cell 76:969.
4. Hill LL, et al. 1999. Science 285:898.
- Gene ID
- 14103 View all products for this Gene ID
- UniProt
- View information about CD178 on UniProt.org
