Ultra-LEAF™ Purified anti-mouse CD178 (FasL)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2184	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, Apop, ICC?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of the cytotoxicity^1-3 and immunofluorescence microscopy⁴. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106604) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 106612).

Application References (PubMed link indicates BioLegend citation)

Fuller CL, et al. 1999. J. Immunol. 162:6337.
Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
Seko Y, et al. 2002. J. Am. Coll.Cardiol. 39:1399.
Park H, et al. 2005. J. Immunol. 175:7193.
Lundgvist A, et al. 2009. Blood 113:6120. PubMed
Oida T, et al. 2011. PLoS ONE 6(4):e18365. (Block) PubMed.
Oura R, et al. 2013. J. Immunol. 190:578. PubMed.

Product Citations

Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed

RRID

AB_2813953 (BioLegend Cat. No. 106611) AB_2813954 (BioLegend Cat. No. 106612)

Antigen Details

Structure: TNF superfamily, 40 kD
Distribution: Activated T cells, spleen, testis, eye
Function: Induces apoptosis
Ligand/Receptor: Fas (CD95)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu?Rev?Genet. 33:209.
3. Takahashi T, et al. 1994. Cell. 76:969.
4. Hill LL, et al. 1999. Science. 285:898.
Gene ID: 14103 View all products for this Gene ID
UniProt: View information about CD178 on UniProt.org

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