首页 > 抗体蛋白 > 抗体
APC anti-mouse CD178 (FasL) Antibody
产品名称:
APC anti-mouse CD178 (FasL) Antibody
产品类别:
抗体
产品编号:
106609
产品应用:
106609
[价格]
规格 价格 库存
25µg ¥ 1428 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of the cytotoxicity1-3 and immunofluorescence microscopy4. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106604) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 106612).

Application References

(PubMed link indicates BioLegend citation)
  1. Fuller CL, et al. 1999. J. Immunol. 162:6337.
  2. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
  3. Seko Y, et al. 2002. J. Am. Coll.Cardiol. 39:1399.
  4. Park H, et al. 2005. J. Immunol. 175:7193.
  5. Lundgvist A, et al. 2009. Blood 113:6120. PubMed
  6. Oida T, et al. 2011. PLoS ONE 6(4):e18365. (Block) PubMed.
  7. Oura R, et al. 2013. J. Immunol. 190:578. PubMed.
Product Citations
  1. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
RRID
AB_2813951 (BioLegend Cat. No. 106609) AB_2813952 (BioLegend Cat. No. 106610)

Antigen Details

Structure
TNF superfamily, 40 kD
Distribution

Activated T cells, spleen, testis, eye

Function
Induces apoptosis
Ligand/Receptor
Fas (CD95)
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu?Rev?Genet. 33:209.
3. Takahashi T, et al. 1994. Cell. 76:969.
4. Hill LL, et al. 1999. Science. 285:898.

Gene ID
14103 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线