Pacific Blue™ anti-mouse FOXP3 Antibody
- 产品名称：
- Pacific Blue™ anti-mouse FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126409
- 产品应用：
- 126409
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Ono M, et al. 2007. Nature 446:685.
- Hori S, et al. 2003. Science 299:1057.
- Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
- Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
- Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
- Product Citations
-
- Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
- Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
- Shears RK, et al. 2020. J Allergy Clin Immunol. 145:1272. PubMed
- Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
- Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
- Pierini A, et al. 2015. J Immunol. 195: 347 - 355. PubMed
- Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
- Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed
- Chen RJ, et al. 2022. iScience. 25:105595. PubMed
- Pandey S, et al. 2014. J Immunol. 193:3632. PubMed
- Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
- Qi J, et al. 2021. Nat Commun. 12:4755. PubMed
- Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
- Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
- Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
- Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
- Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
- Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
- Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
- Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
- RRID
- AB_2247064 (BioLegend Cat. No. 126409) AB_2105047 (BioLegend Cat. No. 126410)
- Structure
- 50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
- Distribution
-
Nuclear; expressed in Treg cells.
- Function
- Master regulatory gene in Treg cell development, crucial for immune homeostasis.
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Regulation
- Present at high level in T reg cells. Induced by T cell activation.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
